| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bifidobacterium animalis | TTTCCAAGGCCACCGCAT | TGAGCTCCTCTTCACGTGC | 59.88 | 59.71 | 153 | 149 |
100.00%
|
100.00%
|
| Primer 2 | Bifidobacterium animalis | ACGCGTGATTCCCGTTGT | AGCGCAAACACCACGATG | 59.97 | 59.05 | 210 | 145 |
100.00%
|
100.00%
|
| Primer 3 | Bifidobacterium animalis | TCGACGATGTGAAGGAGGC | ATCGGCATCATCGGAGAGC | 59.49 | 59.71 | 170 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Bifidobacterium animalis | ATTCGCAAGCGCATCAGC | AGCTCGCCAACCTTCTTGG | 59.59 | 60.30 | 188 | 143 |
100.00%
|
100.00%
|
| Primer 5 | Bifidobacterium animalis | ACGCGTGATTCCCGTTGT | CAGCGCAAACACCACGAT | 59.97 | 59.05 | 211 | 141 |
100.00%
|
100.00%
|
| Primer 6 | Bifidobacterium animalis | CATTGCAATCACCGTGGCC | TGTTCGACCATCTGCGCA | 60.15 | 59.66 | 204 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Bifidobacterium animalis | TGAATTCGAGCGGACCGTC | TGTTCGACCATCTGCGCA | 60.15 | 59.66 | 273 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Bifidobacterium animalis | ACCTGAATTCGAGCGGACC | TGTTCGACCATCTGCGCA | 59.78 | 59.66 | 276 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Bifidobacterium animalis | ATTGCAATCACCGTGGCC | TGTTCGACCATCTGCGCA | 58.71 | 59.66 | 203 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Bifidobacterium animalis | AATTCGAGCGGACCGTCAC | TGTTCGACCATCTGCGCA | 60.45 | 59.66 | 271 | 138 |
100.00%
|
100.00%
|